Contact an Account ManagerSome advantages of high temperature for the separation of pharmaceutical compounds with mass spectrometry detection
Summary
Type: HPLC
Application #: 121470
Catalog#: 760080.20 - MN HPLC Column, Nucleodur C18 Gravity, 110A, 3um, 2x50mm
Detection: LC-ESI-MS (positive mode, capillary needle voltage +4.25 V, nitrogen gas flow 80 a.u (sheath gas) and 20 a.u. (auxillary gas), transfer cappilary temperature 250 °C, full scan mode)
Conditions: Condition (a):
Eluent: A: formic acid in water, pH 2.8, B: acetonitrile
Gradient: 10 to 100% B for 9 min
Flow rate: 0.2 ml/min
Temperature: 30 °C
Condition (b):
Eluent: A: ammonia in water, pH 10.4, B: acetonitrile
Gradient: 10 to 100% B for 9 min
Flow rate: 0.2 ml/min
Temperature: 30 °C
Condition (c):
Eluent: A: ammonia in water, pH 10.4, B: acetonitrile
Gradient: 10 to 100% B for 3 min
Flow rate: 0.6 ml/min
Temperature: 90 °C
Authors: Heinisch, S. et al.
Source: J. Sep. Sci. 2005, 28, 1803-1811